Histological assessment of nanostructured fibrin‐agarose skin substitutes grafted in burnt patients. A time‐course study

A previously developed fibrin-agarose skin model—UGRSKIN—showed promising clinical results in severely burnt patients. To determine the histological parameters associated to biocompatibility and therapeutic effects of this model, we carried out a comprehensive structural ultrastructural study UGRSKIN grafted patients after 3 months follow-up. The epidermis was analogue native human from day 30th onward, revealing well-structured strata with well-differentiated keratinocytes expressing CK5, CK8, CK10, claudin, plakoglobin, filaggrin, involucrin similar way controls, suggesting that able mature differentiate very early. Melanocytes Langerhans cells were found together basement membrane, abundant hemidesmosomes lack rete ridges. At dermal layer, an interface between host tissue at 30th, which tended disappear time. superficial dermis showed progressive increase properly-oriented collagen fibers, elastic fibers proteoglycans, including decorin, similarly control 60-90th vivo Blood vessels determined by CD31 SMA expression more than whereas lymphatic 90th. These contribute shed light on effect model demonstrate bioengineered is early epithelial level 60–90 days level.